bs 23127r  (Bioss)

Journal: PLoS ONE

Article Title: Vitamin D Deficiency Induces High Blood Pressure and Accelerates Atherosclerosis in Mice

doi: 10.1371/journal.pone.0054625

Figure Lengend Snippet: Peritoneal macrophages from ApoE −/− mice after vitamin D-sufficient or –deficient HFD were assessed by flow cytometry for ( A ) Cell surface markers for M1 and M2 phenotype (CCR7: gray, CD86: black, MR: dots, CD163: white) and ( B ) Macrophage phenotype ratio calculated from flow cytometry analysis to assess M1 vs. M2 predominance (vitamin D-sufficient: black, vitamin D-deficient: white). From the aortic root of vitamin D-sufficient (top) and -deficient (bottom) animals after 8 weeks on HFD, ( C ) Represenative image of double immunofluorescent staining for CCR7 (M1, green), MR (M2, green), and ADRP (red). Scale bar represents 50 µm. ( D ) Quantification of CCR7 immunofluorescent staining as a percentage of total atherosclerotic plaque area, ( E ) Co-localization (yellow) of CCR7 and MR with ADRP as a percentage of ADRP-positive area (n = 3 per group for all). Data expressed as mean ± SEM. *p<0.05, **p<0.01.

Article Snippet: Macrophage cell surface marker analysis was performed using a FACStar Plus with PE-conjugated anti-CCR7 and anti-CD86 (1∶100 for both, E-Bioscience) for M1 macrophage membrane protein expression and FITC-conjugated anti-CD163 (1∶20, Bioss USA) and anti-MR (1∶100, R&D Systems) for M2 macrophage membrane protein expression .

Techniques: Flow Cytometry, Staining

Journal: Behavioural Neurology

Article Title: PPAR- γ Promotes Hematoma Clearance through Haptoglobin-Hemoglobin-CD163 in a Rat Model of Intracerebral Hemorrhage

doi: 10.1155/2018/7646104

Figure Lengend Snippet: Effect of Glivec and monascin on PPAR- γ , haptoglobin, and CD163 associated with ICH 3 days after surgery. Representative images are shown of Western blot assay (a–d) and real-time PCR (e and f) for PPAR- γ , haptoglobin, and CD163 levels within ipsilateral brain tissues. One-way ANOVA followed by Tukey's tests was used. ( ∗ p < 0.05 versus sham; # p < 0.05 versus vehicle).

Article Snippet: Membranes were blocked with 5% BSA blocking buffer at 37°C for 2 hours and incubated with first antibodies: polyclonal anti-PPAR- γ of rabbit (1 : 1000, Bioss), anti-CD163 of rabbit (1 : 500, Bioss), and polyclonal anti-Hp of rabbit (1 : 500, Bioss) at 4°C in a thermostat shaker overnight.

Techniques: Western Blot, Real-time Polymerase Chain Reaction

Journal: BMC Cancer

Article Title: Prognostic value of tumor-infiltrating CD163 + macrophage in patients with metastatic gastric cancer undergoing multidisciplinary treatment

doi: 10.1186/s12885-022-09713-y

Figure Lengend Snippet: Comparison of clinicopathological characteristics between non-conversion surgery group and conversion surgery group

Article Snippet: Next, the slides were incubated with primary rabbit polyclonal anti-CD4 antibody (1:300 dilution; Abcam, Cambridge, UK), primary rabbit polyclonal anti-CD8 antibody (1:200 dilution; Abcam), primary mouse monoclonal anti-Foxp3 antibody (1:100 dilution; Abcam), primary rabbit polyclonal anti-CD163 antibody (1:300 dilution; Bioss, Woburn, USA), and primary mouse monoclonal anti-CD68 antibody (1:200 dilution; Abcam) at 4℃ overnight.

Techniques:

Journal: BMC Cancer

Article Title: Prognostic value of tumor-infiltrating CD163 + macrophage in patients with metastatic gastric cancer undergoing multidisciplinary treatment

doi: 10.1186/s12885-022-09713-y

Figure Lengend Snippet: The receiver operating curves of individual parameters. The optimal cut-off levels and areas under the curves of NLR, LMR, PLR, albumin, CD4 + lymphocyte, CD8 + lymhpocte, FoxP3 + lymphocyte, CD68 + macrophage, and CD163 + macrophage were calculated by ROC curve

Article Snippet: Next, the slides were incubated with primary rabbit polyclonal anti-CD4 antibody (1:300 dilution; Abcam, Cambridge, UK), primary rabbit polyclonal anti-CD8 antibody (1:200 dilution; Abcam), primary mouse monoclonal anti-Foxp3 antibody (1:100 dilution; Abcam), primary rabbit polyclonal anti-CD163 antibody (1:300 dilution; Bioss, Woburn, USA), and primary mouse monoclonal anti-CD68 antibody (1:200 dilution; Abcam) at 4℃ overnight.

Techniques:

Journal: BMC Cancer

Article Title: Prognostic value of tumor-infiltrating CD163 + macrophage in patients with metastatic gastric cancer undergoing multidisciplinary treatment

doi: 10.1186/s12885-022-09713-y

Figure Lengend Snippet: The correlation between OS and clinicopathological factors assessable prior to chemotherapy in all patients

Article Snippet: Next, the slides were incubated with primary rabbit polyclonal anti-CD4 antibody (1:300 dilution; Abcam, Cambridge, UK), primary rabbit polyclonal anti-CD8 antibody (1:200 dilution; Abcam), primary mouse monoclonal anti-Foxp3 antibody (1:100 dilution; Abcam), primary rabbit polyclonal anti-CD163 antibody (1:300 dilution; Bioss, Woburn, USA), and primary mouse monoclonal anti-CD68 antibody (1:200 dilution; Abcam) at 4℃ overnight.

Techniques:

Journal: BMC Cancer

Article Title: Prognostic value of tumor-infiltrating CD163 + macrophage in patients with metastatic gastric cancer undergoing multidisciplinary treatment

doi: 10.1186/s12885-022-09713-y

Figure Lengend Snippet: Multivariate analysis of independent risk factor for overall survival in patients who underwent DCS therapy

Article Snippet: Next, the slides were incubated with primary rabbit polyclonal anti-CD4 antibody (1:300 dilution; Abcam, Cambridge, UK), primary rabbit polyclonal anti-CD8 antibody (1:200 dilution; Abcam), primary mouse monoclonal anti-Foxp3 antibody (1:100 dilution; Abcam), primary rabbit polyclonal anti-CD163 antibody (1:300 dilution; Bioss, Woburn, USA), and primary mouse monoclonal anti-CD68 antibody (1:200 dilution; Abcam) at 4℃ overnight.

Techniques:

Journal: BMC Cancer

Article Title: Prognostic value of tumor-infiltrating CD163 + macrophage in patients with metastatic gastric cancer undergoing multidisciplinary treatment

doi: 10.1186/s12885-022-09713-y

Figure Lengend Snippet: Associations between CD163 + macrophages infiltration and clinicopathological variables in all cases

Article Snippet: Next, the slides were incubated with primary rabbit polyclonal anti-CD4 antibody (1:300 dilution; Abcam, Cambridge, UK), primary rabbit polyclonal anti-CD8 antibody (1:200 dilution; Abcam), primary mouse monoclonal anti-Foxp3 antibody (1:100 dilution; Abcam), primary rabbit polyclonal anti-CD163 antibody (1:300 dilution; Bioss, Woburn, USA), and primary mouse monoclonal anti-CD68 antibody (1:200 dilution; Abcam) at 4℃ overnight.

Techniques:

Journal: BMC Cancer

Article Title: Prognostic value of tumor-infiltrating CD163 + macrophage in patients with metastatic gastric cancer undergoing multidisciplinary treatment

doi: 10.1186/s12885-022-09713-y

Figure Lengend Snippet: Kaplan–Meier curves of overall survival according to the infiltration of CD163 + M2 macrophages. a in all patients enrolled in this study b in patients who underwent conversion surgery. Patients with high CD163 + M2 macrophages infiltration had a significantly worse prognosis than those with low CD163 + M2 macrophage infiltration in both all patients and conversion cases

Article Snippet: Next, the slides were incubated with primary rabbit polyclonal anti-CD4 antibody (1:300 dilution; Abcam, Cambridge, UK), primary rabbit polyclonal anti-CD8 antibody (1:200 dilution; Abcam), primary mouse monoclonal anti-Foxp3 antibody (1:100 dilution; Abcam), primary rabbit polyclonal anti-CD163 antibody (1:300 dilution; Bioss, Woburn, USA), and primary mouse monoclonal anti-CD68 antibody (1:200 dilution; Abcam) at 4℃ overnight.

Techniques: